Composite

Part:BBa_K3376009

Designed by: Li, Sheng-Fong   Group: iGEM20_Mingdao   (2020-10-06)


SpxB-Tr/pSB1C3

Pyruvate oxidase (SpxB) of S. sanguinis catalyzes the reaction between pyruvate and oxygen to generate acetyl phosphate and H2O2. It plays a role in antagonistic relationship against S. mutans. The E. coli Nissle carrying SpxB gene is capable of producing 2.15 mM and 1.84 mM of H2O2 per OD600 when grown in 5% and 10% glucose, respectively, compared to the background level of H2O2 production (i.e., 0.33 mM)



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 658
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 577
    Illegal SapI site found at 1255
    Illegal SapI.rc site found at 1711


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